H2N2V1, Main, Exploration, bibRecord, 001455

Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR

Identifieur interne : 001455 ( Main/Exploration ); précédent : 001454; suivant : 001456

Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR

Auteurs : Deepanker Tewari [États-Unis] ; Corey Zellers [États-Unis] ; Helen Acland [États-Unis] ; Janice C. Pedersen [États-Unis]

Source :

Journal of clinical virology [ 1386-6532 ] ; 2007.

RBID : Pascal:07-0480567

Descripteurs français

KwdFr :
- ARN viral (isolement et purification), Animaux, Automatisation, Embryon de poulet, Grippe chez les oiseaux (diagnostic), Magnétisme, Microsphères, Poulets (virologie), Robotique, Réaction de polymérisation en chaîne (), Sensibilité et spécificité, Sous-type H5N1 du virus de la grippe A (génétique), Sous-type H5N1 du virus de la grippe A (isolement et purification), Trachée (virologie).
MESH :
- diagnostic : Grippe chez les oiseaux.
- génétique : Sous-type H5N1 du virus de la grippe A.
- isolement et purification : ARN viral, Sous-type H5N1 du virus de la grippe A.
- virologie : Poulets, Trachée.
Pascal (Inist)
- Animaux, Automatisation, Embryon de poulet, Influenzavirus aviaire, Aves, Détection, Magnétisme, Microsphères, Robotique, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Temps réel, Réaction chaîne polymérase RT, Microbiologie, Virologie, Grippe A.

English descriptors

KwdEn :
- Animals, Automation, Aves, Avian influenzavirus, Chick Embryo, Chickens (virology), Detection, Influenza A, Influenza A Virus, H5N1 Subtype (genetics), Influenza A Virus, H5N1 Subtype (isolation & purification), Influenza in Birds (diagnosis), Magnetics, Microbiology, Microspheres, Polymerase Chain Reaction (methods), RNA, Viral (isolation & purification), Real time, Reverse transcription polymerase chain reaction, Robotics, Sensitivity and Specificity, Trachea (virology), Virology.
MESH :
- chemical , isolation & purification : RNA, Viral.
- diagnosis : Influenza in Birds.
- genetics : Influenza A Virus, H5N1 Subtype.
- isolation & purification : Influenza A Virus, H5N1 Subtype.
- methods : Polymerase Chain Reaction.
- virology : Chickens, Trachea.
- Animals, Automation, Chick Embryo, Magnetics, Microspheres, Robotics, Sensitivity and Specificity.

Abstract

Background: Highly pathogenic H5N1 avian influenza (AI) poses a grave risk to human health. An important aspect of influenza control is rapid diagnosis. Objectives: This study describes the efficiency of AI-RNA extraction utilizing silica-based magnetic beads with robotics and its detection with an influenza A matrix gene real-time RT-PCR from tracheal swabs, and compares it to virus isolation and manual spin column extractions. Study design: Analytical sensitivity was assessed by performing dilution analysis and detection of H2N2 AI viral RNA. Diagnostic sensitivity and specificity was assessed by analyzing tracheal swabs collected from H7N2 infected and uninfected chickens. Results: Both manual and robotic extractions detected AI virus at 1 log₁₀ EID₅₀/ml. Diagnostic sensitivity and specificity of matrix gene detection with the automated extraction method for chicken tracheal swab specimens was similar to that of virus isolation and the manual extraction method. There were only three discordant results among 212 tested specimens. Conclusion: The main advantages of automated robotic viral nucleic acid extraction are high throughput processing; hands-free operation; and reduction in human and technical error. This study demonstrates successful detection of influenza A virus with magnetic beads utilizing the Qiagen MagAttract cell kit on a BioRobot M48 platform.

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR</title>
<author><name sortKey="Tewari, Deepanker" sort="Tewari, Deepanker" uniqKey="Tewari D" first="Deepanker" last="Tewari">Deepanker Tewari</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Microbiology Services, Pennsylvania Veterinary Laboratory, 2305 N. Cameron St</s1>
<s2>Harrisburg, PA 17110</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zellers, Corey" sort="Zellers, Corey" uniqKey="Zellers C" first="Corey" last="Zellers">Corey Zellers</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Microbiology Services, Pennsylvania Veterinary Laboratory, 2305 N. Cameron St</s1>
<s2>Harrisburg, PA 17110</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Acland, Helen" sort="Acland, Helen" uniqKey="Acland H" first="Helen" last="Acland">Helen Acland</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Microbiology Services, Pennsylvania Veterinary Laboratory, 2305 N. Cameron St</s1>
<s2>Harrisburg, PA 17110</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pedersen, Janice C" sort="Pedersen, Janice C" uniqKey="Pedersen J" first="Janice C." last="Pedersen">Janice C. Pedersen</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Diagnostic Virology Laboratory, National Veterinary Services Laboratories, 1800 Dayton Road</s1>
<s2>Ames, IA</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Iowa</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">07-0480567</idno>
<date when="2007">2007</date>
<idno type="stanalyst">PASCAL 07-0480567 INIST</idno>
<idno type="RBID">Pascal:07-0480567</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000037</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000037</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000039</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000039</idno>
<idno type="wicri:doubleKey">1386-6532:2007:Tewari D:automated:extraction:of</idno>
<idno type="wicri:Area/Main/Merge">001474</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:17875401</idno>
<idno type="wicri:Area/PubMed/Corpus">000272</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000272</idno>
<idno type="wicri:Area/PubMed/Curation">000272</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000272</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000264</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000264</idno>
<idno type="wicri:Area/Ncbi/Merge">000245</idno>
<idno type="wicri:Area/Ncbi/Curation">000245</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000245</idno>
<idno type="wicri:doubleKey">1386-6532:2007:Tewari D:automated:extraction:of</idno>
<idno type="wicri:Area/Main/Merge">001410</idno>
<idno type="wicri:Area/Main/Curation">001455</idno>
<idno type="wicri:Area/Main/Exploration">001455</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR</title>
<author><name sortKey="Tewari, Deepanker" sort="Tewari, Deepanker" uniqKey="Tewari D" first="Deepanker" last="Tewari">Deepanker Tewari</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Microbiology Services, Pennsylvania Veterinary Laboratory, 2305 N. Cameron St</s1>
<s2>Harrisburg, PA 17110</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zellers, Corey" sort="Zellers, Corey" uniqKey="Zellers C" first="Corey" last="Zellers">Corey Zellers</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Microbiology Services, Pennsylvania Veterinary Laboratory, 2305 N. Cameron St</s1>
<s2>Harrisburg, PA 17110</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Acland, Helen" sort="Acland, Helen" uniqKey="Acland H" first="Helen" last="Acland">Helen Acland</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Microbiology Services, Pennsylvania Veterinary Laboratory, 2305 N. Cameron St</s1>
<s2>Harrisburg, PA 17110</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pedersen, Janice C" sort="Pedersen, Janice C" uniqKey="Pedersen J" first="Janice C." last="Pedersen">Janice C. Pedersen</name>
<affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Diagnostic Virology Laboratory, National Veterinary Services Laboratories, 1800 Dayton Road</s1>
<s2>Ames, IA</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Iowa</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of clinical virology</title>
<title level="j" type="abbreviated">J. clin. virol.</title>
<idno type="ISSN">1386-6532</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of clinical virology</title>
<title level="j" type="abbreviated">J. clin. virol.</title>
<idno type="ISSN">1386-6532</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Automation</term>
<term>Aves</term>
<term>Avian influenzavirus</term>
<term>Chick Embryo</term>
<term>Chickens (virology)</term>
<term>Detection</term>
<term>Influenza A</term>
<term>Influenza A Virus, H5N1 Subtype (genetics)</term>
<term>Influenza A Virus, H5N1 Subtype (isolation & purification)</term>
<term>Influenza in Birds (diagnosis)</term>
<term>Magnetics</term>
<term>Microbiology</term>
<term>Microspheres</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>Real time</term>
<term>Reverse transcription polymerase chain reaction</term>
<term>Robotics</term>
<term>Sensitivity and Specificity</term>
<term>Trachea (virology)</term>
<term>Virology</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (isolement et purification)</term>
<term>Animaux</term>
<term>Automatisation</term>
<term>Embryon de poulet</term>
<term>Grippe chez les oiseaux (diagnostic)</term>
<term>Magnétisme</term>
<term>Microsphères</term>
<term>Poulets (virologie)</term>
<term>Robotique</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Sensibilité et spécificité</term>
<term>Sous-type H5N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H5N1 du virus de la grippe A (isolement et purification)</term>
<term>Trachée (virologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza in Birds</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe chez les oiseaux</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poulets</term>
<term>Trachée</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chickens</term>
<term>Trachea</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Automation</term>
<term>Chick Embryo</term>
<term>Magnetics</term>
<term>Microspheres</term>
<term>Robotics</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Automatisation</term>
<term>Embryon de poulet</term>
<term>Influenzavirus aviaire</term>
<term>Aves</term>
<term>Détection</term>
<term>Magnétisme</term>
<term>Microsphères</term>
<term>Robotique</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Temps réel</term>
<term>Réaction chaîne polymérase RT</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Grippe A</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Background: Highly pathogenic H5N1 avian influenza (AI) poses a grave risk to human health. An important aspect of influenza control is rapid diagnosis. Objectives: This study describes the efficiency of AI-RNA extraction utilizing silica-based magnetic beads with robotics and its detection with an influenza A matrix gene real-time RT-PCR from tracheal swabs, and compares it to virus isolation and manual spin column extractions. Study design: Analytical sensitivity was assessed by performing dilution analysis and detection of H2N2 AI viral RNA. Diagnostic sensitivity and specificity was assessed by analyzing tracheal swabs collected from H7N2 infected and uninfected chickens. Results: Both manual and robotic extractions detected AI virus at 1 log<sub>10</sub>
 EID<sub>50</sub>
/ml. Diagnostic sensitivity and specificity of matrix gene detection with the automated extraction method for chicken tracheal swab specimens was similar to that of virus isolation and the manual extraction method. There were only three discordant results among 212 tested specimens. Conclusion: The main advantages of automated robotic viral nucleic acid extraction are high throughput processing; hands-free operation; and reduction in human and technical error. This study demonstrates successful detection of influenza A virus with magnetic beads utilizing the Qiagen MagAttract cell kit on a BioRobot M48 platform.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Iowa</li>
<li>Pennsylvanie</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Tewari, Deepanker" sort="Tewari, Deepanker" uniqKey="Tewari D" first="Deepanker" last="Tewari">Deepanker Tewari</name>
</region>
<name sortKey="Acland, Helen" sort="Acland, Helen" uniqKey="Acland H" first="Helen" last="Acland">Helen Acland</name>
<name sortKey="Pedersen, Janice C" sort="Pedersen, Janice C" uniqKey="Pedersen J" first="Janice C." last="Pedersen">Janice C. Pedersen</name>
<name sortKey="Zellers, Corey" sort="Zellers, Corey" uniqKey="Zellers C" first="Corey" last="Zellers">Corey Zellers</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001455 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001455 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:07-0480567
   |texte=   Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

Serveur d'exploration H2N2

Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR

Automated extraction of avian influenza virus for rapid detection using real-time RT-PCR

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.